Description efficient gas delivery while minimizing excess gas exposure to lab personnel. Four superoxide dismutases contribute to bacillus anthracis. Always adhere to proper diameter index safety system d. Hematopoietic stem cell protocols pdf free download. Using the xgi8 gas anesthesia system university of houston. Select a user id from the dropdown list or enter a new user id up to three letters, and click ok 7. Xgi8 gas anesthesia system for ivis preclinical in vivo. Pdf secreted luciferase for in vivo evaluation of systemic. Buy with confidence, your full satisfaction is guaranteed. The gas anesthesia module successfully integrates e. Secreted luciferase for in vivo evaluation of systemic.
Use only oxygen regulated to 5055 psi as the carrier gas. Secreted luciferase for in vivo evaluation of systemic protein delivery in mice. The xgi 8 gas anesthesia system is intended for use with ivis imaging systems to anesthetize up to 10 laboratory mice prior to and during imaging. Xgi8 gas anesthesia system how to exchange the vaporizer. Perkinelmers xgi8 gas anesthesia system is designed to work with ivis imaging systems and allows researchers to use realtime. Pleasanton, ca usa 8004666463 5 for veterinary use only. Inspectturns the fluorescent lamp on manually, allowing the user to inspect the action inside the ivis. Initialize the system, wait for the camera to cool down till the temperature button turns green. Compositions and methods for inducing nanoparticle. For intranasal spore challenge, mice were sedated with isoflurane with an xgi8 gas anesthesia system xenogen corporation.
Mice or rats are anesthesized in the induction chamber prior to imaging. The standard mounting system requires bolting of the vaporizer directly to a rigid back bar of an anesthesia gas machine. Personnel operating this compac5 must be thoroughly familiar with the instruction manual and equipment operation prior to use with patients. The ivis200 bioluminescence imaging machine was equipped with an xgi8 gas anesthesia system, and isoflurane butler schein, 029404 with a flow rate of 2. Xenogen gas anesthesia system model xgi 8 with warranty. Caliper life sciences xenogen xgi8 community, manuals. The xgi8 gas anesthesia system delivers and actively scavenges gas during the imaging process. The xenogen xgi8 gas anesthesia system is designed to work with the ivis imaging system, a technology from xenogen that allows researchers to use r. If it is not already on, start up the acquisition computer and start the. It facilitates efficient gas delivery via four delivery circuits while minimizing excess gas exposure to lab personnel with active scavenging capabilities.
Mar 11, 2012 secreted luciferase for in vivo evaluation of systemic protein delivery in mice. This protocol describes a methodology for imaging the sequestration of infected erythrocytes of the rodent malaria parasite plasmodium berghei in. Xaf8 anesthesia system filters 8 pk118999perkinelmer. Perkinelmer caliper ivis spectrum hardware manual pdf download. Animals were injected intraperitoneally 5 minutes prior to imaging with luciferin 150mgkg of body weight, 1mg dluciferin in 1ml of 2x pbs, xenogen, xr1001. We specialize in buying and selling high quality, new and used laboratory and pharmaceutical equipment. Xgi8 gas anesthesia isoflurane vaporizer with independent flow control for proper gas delivery to induction chamber and the ivis imaging system imaging chamber isofluraneabsorbing disposable charcoal filters to absorb excess gas, as well as limit gas escaping into the surrounding laboratory environment. The anesthesia system allows researchers to use realtime. The xenogen xgi8 gas anesthesia system is designed to work with the ivis imaging system, a technology from xenogen that allows researchers to use realtime in vivo imaging to monitor and record cellular and genetic activity within a living organism. The xgi 8 anesthesia module is the main device that controls the delivery of. The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense. Nanoparticle mediated microvascular embolization nme of tumor tissue may occur after systemic administration of pem, leading to widespread shutdown of vascular flow, hemorrhage, and necrosis. For that purpose, we constructed a reporter plasmid with the lux operon under control of the spore small acidsoluble protein b sspb promoter.
Using the xgi8 gas anesthesia system bbic university of houston. Perkinelmers xgi8 gas anesthesia system is designed to work with ivis. Unless otherwise specified, all vaporizers are supplied standard with 23mm inlet and outlet tapers. Xgi8 gas anesthesia system for ivis preclinical in.
This protein is upregulated in host cells under hypoxic conditions and thus can be used as a marker of oxygen levels present in tissues. Xgi8 gas anesthesia system contact information please call 1. Tampering with the anesthesia system components by unauthorized personnel voids all warranties and specifications. Prior to imaging, the mice were anesthetized using the xgi8 gas anesthesia system caliper life sciences, inc.
Imaging was performed using an ivis spectrum optical imaging system fitted with an xgi8 gas anesthesia system caliper life sciences, hopkinton, ma. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politics. The gas anesthesia module successfully integrates efficient gas delivery while minimizing. This instrument may also be used for fluorescent imaging of gels or cell culture plates. See page 31 for more details on the use of the transfer bed and mouse imaging shuttle. Hypoxisense is a fluorescent dye which detects surface expression of carboxic anhydrase 9. Bioluminescence imaging was obtained by acquiring photon counts over 5 min immediately after intravenous tailvein injection of 150 l coelenterazine 4 gg body weight using a 26 mm square backthinned ccd camera.
The xgi8 gas anesthesia system is intended for use with ivis imaging systems to anesthetize up to 10 laboratory mice prior to and during imaging. The xgi8 system continuously removes the waste anesthesia gas mixture from the area around the anesthetized mice to reduce, though not eliminate, user exposure to the isoflurane anesthetic agent. The ivis imaging system 100 series and the xgi8 gas anesthesia system. Before turning on the xgi8 anesthesia machine it is important to checkdo the following weigh the fair canisters. Welcome camera xgi8 gas anesthesia system high resolution monitor imaging chamber acquisition computer xws260 workstation compartment that contains the thermocube chiller unit and camera power supply figure 1. Living lmage software version 2 cornell university. The ras4 rodent anesthesia system is compatible with our suite of in vivo preclinical imaging systems including ivis and fmt optical imaging systems as well as the quantum microct imaging system.
Before turning on the xgi8 anesthesia machine it is important to checkdo the following. Ivis spectrum 7 ivis spectrum complete imaging solutions accessories reagents anesthesia system cat no. The ivis200 bioluminescence imaging machine was equipped with an xgi 8 gas anesthesia system, and isoflurane butler schein, 029404 with a flow rate of 2. If you have questions regarding this manual or the ivis imaging system, please. The xenogen xgi8 gas anesthesia system is designed to work with the ivis imaging system, a technology from xenogen that allows researchers to use r ealtime in vivo imaging to monitor and record cellular and genetic activity within a living organism. The xgi8 anesthesia module is the main device that controls the delivery of. Hematopoietic stem cell protocols m e t h o d s i n m o l e c u l a r b i o l o g ytm john m. An integrated gas isoflurane anesthesia xgi8 anesthesia system, allows for repeated daily use in rodents. Mice were under continuous anesthesia on a heated stage using the xgi8 gas anesthesia system.
Ivis spectrum imaging core research technology support facility. Gas anesthesia ports and 3 or 5 position manifold within imaging chamber allow anesthesia to be maintained during imaging sessions syringe injection system, integrated with living image, allows the user to acquire real time functional responses to compounds ivis lumina xr a b a b exchangeable filter wheel shielded cabinet xray module. The xgi 8 gas anesthesia system delivers and then minimizes exposure to excess gas during the imaging process. Caliper recommends using the xgi8 gas anesthesia system when imaging small animals. The xgi 8 system continuously removes the waste anesthesia gas mixture from the area around the anesthetized mice to reduce, though not eliminate, user exposure to the isoflurane anesthetic agent.
Imaging usc school of pharmacy core facilities usc. Click on our partners to see what last minute vacation destinations they airline jobs in massachusetts for the how to study for mrcp part 1 three years, sunwing has been listed by profit magazine as one of. The xgi8 system continuously removes the waste anesthesia gas mixture from the area around the anesthetized mice to reduce, though not eliminate, user. Ivis spectrum imaging core research technology support. Vetequip assumes no liability for any malfunction, failure, damage or loss to either equipment or life. A lighttight imaging chamber for quantitative bioluminescent and fluorescent imaging in vivo and in vitro. Computational vision and medical image processing pdf. The ras4 successfully facilitates efficient gas delivery via four delivery circuits. Distributor agreement between xenogen corporation 860.
Realtime in vivo imaging of transgenic bioluminescent. We sought to visualize the site of bacillus anthracis spore germination in vivo. The xgi8 anesthesia system is used in conjunction with the ivis spectrum to anesthetize mice undergoing bioluminescent or fluorescent whole body imaging. The xenogen xgi8 gas anesthesia system is designed to work with the ivis imaging system, a technology from xenogen that allows researchers to use realtime in vivo imaging to monitor and record cellular and genetic. Perkinelmer s xgi8 gas anesthesia system is designed to work with ivis imaging systems and allows researchers to use realtime. Perkinelmer caliper ivis spectrum hardware manual pdf. Avian influenza virus, edited by erica spackman, 2008 435. Hoggan scientific microfet2 handheld manual muscle tester kit w warranty. The cornerstone of the innate immune response is the diverse group of cells, including macrophages, neutrophils, and lymphocytes, that contribute to host defense through the recognition, isolation, and eradication of pathogens. Ensure adequate ventilation before using gas anesthesia equipment.
Introduction to the ibox anesthesia system the ibox anesthesia system is designed for the anesthesia of small animals. Caliper life sciences mobilityshift optimization perkinelmer caliper life sciences mobilityshift optimization. Chromosomal mutagenesis, edited by greg davis and kevin j. The xgi 8 anesthesia system is used in conjunction with the ivis spectrum to anesthetize mice undergoing bioluminescent or fluorescent whole body imaging. Caliper recommends using the xgi 8 gas anesthesia system when imaging small animals. Welcome 4 the system takes very lowlight leve l images, stores them, and displays. Detection of bacillus anthracis spore germination in vivo. An integrated gas isoflurane anesthesia xgi 8 anesthesia system, allows for repeated daily use in rodents.
The xgi8 gas anesthesia system is designed to work with ivis imaging systems and allows researchers to use realtime in vivo imaging to monitor and record cellular and genetic activity within a living organism the gas anesthesia module successfully integrates efficient gas. Do not touch or expose the four diffusing reflectors and the exposed emission filter to contaminants described in system components, page 35 and shown in figure 9. Place the animals in the induction box 6, and wait till the desired level of anesthesia is reached. Efficient gas delivery while minimizing excess gas exposure to lab personnel. Heatactivated spore suspensions of various concentrations see below in 25.
Turn on the flow of gas to the ivis manifold with toggle 7. The system is a small portable unit that is designed for maximum efficiency, ease of use and low anesthetic gas consumption. Caliper life sciences xenogen xgi8 by caliper life sciences. A heated stage, to help maintain body temperature during imaging. These isofluraneabsorbing charcoal filters are made for use with xgi8 anesthesia system. Computational vision and medical image processing pdf free. Caliper life sciences xenogen xgi8 community, manuals and. It facilitates efficient gas delivery via four delivery circuits while minimizing excess gas exposure to lab personnel with active scavenging. Prior to imaging, the mice were anesthetized using the xgi 8 gas anesthesia system caliper life sciences, inc.
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